Researchers at the Universities of York and Torino have used mathematics as a tool to provide precise details of the structure of protein nanoparticles, potentially making them more useful in vaccine design.
Working with a team at the University of Connecticut that focused on the development of self-assembling protein nanoparticles (SAPNs) for vaccine design, they have used advanced mathematical calculations to create a complete picture of the surface morphology of these particles. The study (“Principles Governing the Self-Assembly of Coiled-Coil Nanoparticles”) is published in the Biophysical Journal.
The nanoparticles self-assemble symmetrically using protein building blocks to create cage or shell-like architectures, which serve a range of functions such as storage, catalysis and structural scaffolding, or as enclosures for viral genomes. But electron microscopy and neutron scattering data has limited effectiveness for researchers attempting to classify the morphology of the nanoparticles, according to the scientists. Using mathematics to predict the geometries of nanoparticles can help scientists to select those whose structures are the most advantageous for the design of new vaccines.
The new study focused on a class of artificial SAPNs designed by Peter Burkhard, Ph.D., a structural biophysicist at the University of Connecticut. When chemically attached to antigens from pathogens, nanoparticles can create simple, potent and cost-effective vaccines. Clinical tests on a malaria vaccine designed in this way are due to start soon.
The York and Torino team, led by biophysicist Reidun Twarock, Ph.D., of the University of York’s York Center for Complex Systems Analysis and the departments of mathematics and biology, used a mathematical tool called tiling theory to predict the symmetric classification of different particle morphologies of SAPNs. They adapted the tiling approach Dr. Twarock previously pioneered in the context of virology to model protein nanoparticles with a mixture of local five- and three-fold symmetry axes.
“We have developed a mathematical approach that allows you to identify the surface structures of these nanoparticles that you cannot get from experimentation alone. Mathematics plays an important role here because it acts like a microscope and helps to give researchers insights they couldn’t get experimentally,” said Dr. Twarock
“The protein nanoparticles show great promise as future vaccine carriers and our malaria vaccine will be tested in a clinical setting within the next year. Understanding the geometric principles of the self-assembly to nanoparticles is essential for the successful design and development as vaccines,” added Dr. Burkhard.